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animal detection  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co animal detection
    Animal Detection, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/detection+probe/Animal+Detection+Probe+Super+Premix/10__1186_slash_s12917___026___05828___7-63-64-72
    Average 92 stars, based on 12 article reviews
    animal detection - by Bioz Stars, 2026-11
    92/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Diagnostic Value of Cross-priming Amplification Combined With CRISPR-Cas12b in Detecting Cell-free DNA in Tuberculous Pleural Effusion
    Article Snippet: .. The following were added for each PCR reaction: 4 μL of cfDNA, 5 μL of Animal Detection U + Probe qPCR Super PreMix (Nanjing Vazyme Biotech Co, Ltd), 0.3 μL each of forward and reverse primer (at a 10μM concentration), and 0.4 μL of the probe (at a 10μM concentration). .. The following were added for each PCR reaction: 4 μL of cfDNA, 5 μL of Animal Detection U + Probe qPCR Super PreMix (Nanjing Vazyme Biotech Co, Ltd), 0.3 μL each of forward and reverse primer (at a 10μM concentration), and 0.4 μL of the probe (at a 10μM concentration).

    Article Title: Development and evaluation of multiplex real-time PCR for rapid identifying major pathogenic mycobacteria from pulmonary and extrapulmonary clinical samples in eastern China
    Article Snippet: .. Multiplex PCR amplification was conducted in a total volume of 10 μL, including 5 μL of 2 × Animal Detection U + Probe qPCR Super PreMix (Nanjing VAzyme Biotech Co., Ltd, China), 3.2 μL of primer/probe mix, and 1.8 μL of DNA template. .. PCR and fluorescence detection were performed using the Bio-Rad CFX96 deep well real-time system (Bio-Rad Laboratories, USA).

    Article Title: Rapid and visual differentiation of pseudorabies virus vaccine and wild-type strains using an RPA-LFD assay
    Article Snippet: .. The PCR thermal profile was as follows: initial denaturation at 98 °C for 2 min; 35 cycles of 98 °C for 10 s, 52 °C for 30 s, 72 °C for 20 s; final extension at 72 °C for 1 min. quantitative real-time PCR (qPCR) was performed according to the method described by Quan et al.24, with a 25 μL reaction system prepared using Animal Detection U+ Probe qPCR Super PreMix (QV114-01, Vazyme, Nanjing, Jiangsu, China). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Diagnostic Value of Cross-priming Amplification Combined With CRISPR-Cas12b in Detecting Cell-free DNA in Tuberculous Pleural Effusion
    Article Snippet: .. The following were added for each PCR reaction: 4 μL of cfDNA, 5 μL of Animal Detection U + Probe qPCR Super PreMix (Nanjing Vazyme Biotech Co, Ltd), 0.3 μL each of forward and reverse primer (at a 10μM concentration), and 0.4 μL of the probe (at a 10μM concentration). .. The following were added for each PCR reaction: 4 μL of cfDNA, 5 μL of Animal Detection U + Probe qPCR Super PreMix (Nanjing Vazyme Biotech Co, Ltd), 0.3 μL each of forward and reverse primer (at a 10μM concentration), and 0.4 μL of the probe (at a 10μM concentration).

    Article Title: Development and evaluation of multiplex real-time PCR for rapid identifying major pathogenic mycobacteria from pulmonary and extrapulmonary clinical samples in eastern China
    Article Snippet: .. Multiplex PCR amplification was conducted in a total volume of 10 μL, including 5 μL of 2 × Animal Detection U + Probe qPCR Super PreMix (Nanjing VAzyme Biotech Co., Ltd, China), 3.2 μL of primer/probe mix, and 1.8 μL of DNA template. .. PCR and fluorescence detection were performed using the Bio-Rad CFX96 deep well real-time system (Bio-Rad Laboratories, USA).

    Article Title: Partial unidirectional translocation from 5AL to 7BS leads to dense spike in an EMS-induced wheat mutant
    Article Snippet: .. Amplification and fluorescence detection were performed using AceQ ® U + Probe Master Mix (Q113, Vazyme, Nanjing, China) with a CFX96 real-time PCR system (CFX96, Bio-Rad Laboratories, Hercules, California, USA). .. Amplification and fluorescence detection were performed using AceQ U + Probe Master Mix (Q113, Vazyme, Nanjing, China) with a CFX96 real-time PCR system (CFX96, Bio-Rad Laboratories, Hercules, California, USA).

    Article Title: Rapid and visual differentiation of pseudorabies virus vaccine and wild-type strains using an RPA-LFD assay
    Article Snippet: .. The PCR thermal profile was as follows: initial denaturation at 98 °C for 2 min; 35 cycles of 98 °C for 10 s, 52 °C for 30 s, 72 °C for 20 s; final extension at 72 °C for 1 min. quantitative real-time PCR (qPCR) was performed according to the method described by Quan et al.24, with a 25 μL reaction system prepared using Animal Detection U+ Probe qPCR Super PreMix (QV114-01, Vazyme, Nanjing, Jiangsu, China). ..

    Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU cell proliferation kit Beyotime Cat#C0078S FerroOrange assay kit Dojindo Cat#F374 Hiscript III reverse transcriptase kit Vazyme Cat#R302-01 Matrigel matrix Corning Cat#356234 Mitochondrial membrane potential assay kit Beyotime Cat#C2006 Mouse TNF-α ELISA kit Boster Cat#EK0527 Mouse IL-4 ELISA kit Boster Cat#EK0405 Mouse IFN-γ ELISA kit Boster Cat#EK0375 Mouse CCL5 ELISA kit NeoBioscience Cat#EMC106 Mouse CCL22 ELISA kit NeoBioscience Cat#EMC105 Mouse bone marrow neutrophil isolation kit Tbdscience Cat#TBD2013NM Nuclear and cytoplasmic protein extraction kit Beyotime Cat#P0027 Reactive oxygen species assay kit Beyotime Cat#S0033S Taurine detection assay kit Cell Biolabs Cat#MET-5071 Taq Pro U+ multiple probe qPCR mix kit Vazyme CatQN213-01 Deposited data Metabolomics data This paper Mendeley data: https://data.mendeley. com/datasets/hy5v62xxpm/2 RNA sequencing This paper SRA: SRP668579 Experimental models: Cell lines HL-60 ATCC Cat#CCL-240 HUVEC ATCC Cat#CRL-1730 Experimental models: Organisms/strains Bama mini pigs Hubei Aofei Biotechnology N/A C57BL/6 mice Hubei Biont Biological Technology N/A Oligonucleotides Primers for qPCR This paper N/A siRNA sequence This paper N/A Recombinant DNA pcDNA3.1-ZNF460 GeneChem N/A pcDNA3.1-GGT1 GeneChem N/A pcDNA3.1-KLF9 GeneChem N/A pcDNA3.1-PGC1α GeneChem N/A Software and algorithms AutoDock Vina Scripps Version 1.1.2 Cutadapt Illumina Version 1.15 FlowJo BD Biosciences Version 10.8.1 GraphPad Prism GraphPad Version 9.5.1 ImageJ NIH Version 1.54f OriginPro 2025 OriginLab Version 10.2.0.188 Pymol Schrödinger Version 2.3.4 Cell Reports Medicine 7, 102714, April 21, 2026 e3 Article ll OPEN ACCESS Medical College, Huazhong University of Science and Technology (Permit number: UHCT-IEC-SOP-016-03-01) and carried out in accordance with the Declaration of Helsinki. ..

    Concentration Assay:

    Article Title: Diagnostic Value of Cross-priming Amplification Combined With CRISPR-Cas12b in Detecting Cell-free DNA in Tuberculous Pleural Effusion
    Article Snippet: .. The following were added for each PCR reaction: 4 μL of cfDNA, 5 μL of Animal Detection U + Probe qPCR Super PreMix (Nanjing Vazyme Biotech Co, Ltd), 0.3 μL each of forward and reverse primer (at a 10μM concentration), and 0.4 μL of the probe (at a 10μM concentration). .. The following were added for each PCR reaction: 4 μL of cfDNA, 5 μL of Animal Detection U + Probe qPCR Super PreMix (Nanjing Vazyme Biotech Co, Ltd), 0.3 μL each of forward and reverse primer (at a 10μM concentration), and 0.4 μL of the probe (at a 10μM concentration).

    Multiplex Assay:

    Article Title: Development and evaluation of multiplex real-time PCR for rapid identifying major pathogenic mycobacteria from pulmonary and extrapulmonary clinical samples in eastern China
    Article Snippet: .. Multiplex PCR amplification was conducted in a total volume of 10 μL, including 5 μL of 2 × Animal Detection U + Probe qPCR Super PreMix (Nanjing VAzyme Biotech Co., Ltd, China), 3.2 μL of primer/probe mix, and 1.8 μL of DNA template. .. PCR and fluorescence detection were performed using the Bio-Rad CFX96 deep well real-time system (Bio-Rad Laboratories, USA).

    Amplification:

    Article Title: Development and evaluation of multiplex real-time PCR for rapid identifying major pathogenic mycobacteria from pulmonary and extrapulmonary clinical samples in eastern China
    Article Snippet: .. Multiplex PCR amplification was conducted in a total volume of 10 μL, including 5 μL of 2 × Animal Detection U + Probe qPCR Super PreMix (Nanjing VAzyme Biotech Co., Ltd, China), 3.2 μL of primer/probe mix, and 1.8 μL of DNA template. .. PCR and fluorescence detection were performed using the Bio-Rad CFX96 deep well real-time system (Bio-Rad Laboratories, USA).

    Article Title: Partial unidirectional translocation from 5AL to 7BS leads to dense spike in an EMS-induced wheat mutant
    Article Snippet: .. Amplification and fluorescence detection were performed using AceQ ® U + Probe Master Mix (Q113, Vazyme, Nanjing, China) with a CFX96 real-time PCR system (CFX96, Bio-Rad Laboratories, Hercules, California, USA). .. Amplification and fluorescence detection were performed using AceQ U + Probe Master Mix (Q113, Vazyme, Nanjing, China) with a CFX96 real-time PCR system (CFX96, Bio-Rad Laboratories, Hercules, California, USA).

    Fluorescence:

    Article Title: Partial unidirectional translocation from 5AL to 7BS leads to dense spike in an EMS-induced wheat mutant
    Article Snippet: .. Amplification and fluorescence detection were performed using AceQ ® U + Probe Master Mix (Q113, Vazyme, Nanjing, China) with a CFX96 real-time PCR system (CFX96, Bio-Rad Laboratories, Hercules, California, USA). .. Amplification and fluorescence detection were performed using AceQ U + Probe Master Mix (Q113, Vazyme, Nanjing, China) with a CFX96 real-time PCR system (CFX96, Bio-Rad Laboratories, Hercules, California, USA).

    Reverse Transcription:

    Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU cell proliferation kit Beyotime Cat#C0078S FerroOrange assay kit Dojindo Cat#F374 Hiscript III reverse transcriptase kit Vazyme Cat#R302-01 Matrigel matrix Corning Cat#356234 Mitochondrial membrane potential assay kit Beyotime Cat#C2006 Mouse TNF-α ELISA kit Boster Cat#EK0527 Mouse IL-4 ELISA kit Boster Cat#EK0405 Mouse IFN-γ ELISA kit Boster Cat#EK0375 Mouse CCL5 ELISA kit NeoBioscience Cat#EMC106 Mouse CCL22 ELISA kit NeoBioscience Cat#EMC105 Mouse bone marrow neutrophil isolation kit Tbdscience Cat#TBD2013NM Nuclear and cytoplasmic protein extraction kit Beyotime Cat#P0027 Reactive oxygen species assay kit Beyotime Cat#S0033S Taurine detection assay kit Cell Biolabs Cat#MET-5071 Taq Pro U+ multiple probe qPCR mix kit Vazyme CatQN213-01 Deposited data Metabolomics data This paper Mendeley data: https://data.mendeley. com/datasets/hy5v62xxpm/2 RNA sequencing This paper SRA: SRP668579 Experimental models: Cell lines HL-60 ATCC Cat#CCL-240 HUVEC ATCC Cat#CRL-1730 Experimental models: Organisms/strains Bama mini pigs Hubei Aofei Biotechnology N/A C57BL/6 mice Hubei Biont Biological Technology N/A Oligonucleotides Primers for qPCR This paper N/A siRNA sequence This paper N/A Recombinant DNA pcDNA3.1-ZNF460 GeneChem N/A pcDNA3.1-GGT1 GeneChem N/A pcDNA3.1-KLF9 GeneChem N/A pcDNA3.1-PGC1α GeneChem N/A Software and algorithms AutoDock Vina Scripps Version 1.1.2 Cutadapt Illumina Version 1.15 FlowJo BD Biosciences Version 10.8.1 GraphPad Prism GraphPad Version 9.5.1 ImageJ NIH Version 1.54f OriginPro 2025 OriginLab Version 10.2.0.188 Pymol Schrödinger Version 2.3.4 Cell Reports Medicine 7, 102714, April 21, 2026 e3 Article ll OPEN ACCESS Medical College, Huazhong University of Science and Technology (Permit number: UHCT-IEC-SOP-016-03-01) and carried out in accordance with the Declaration of Helsinki. ..

    Membrane:

    Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU cell proliferation kit Beyotime Cat#C0078S FerroOrange assay kit Dojindo Cat#F374 Hiscript III reverse transcriptase kit Vazyme Cat#R302-01 Matrigel matrix Corning Cat#356234 Mitochondrial membrane potential assay kit Beyotime Cat#C2006 Mouse TNF-α ELISA kit Boster Cat#EK0527 Mouse IL-4 ELISA kit Boster Cat#EK0405 Mouse IFN-γ ELISA kit Boster Cat#EK0375 Mouse CCL5 ELISA kit NeoBioscience Cat#EMC106 Mouse CCL22 ELISA kit NeoBioscience Cat#EMC105 Mouse bone marrow neutrophil isolation kit Tbdscience Cat#TBD2013NM Nuclear and cytoplasmic protein extraction kit Beyotime Cat#P0027 Reactive oxygen species assay kit Beyotime Cat#S0033S Taurine detection assay kit Cell Biolabs Cat#MET-5071 Taq Pro U+ multiple probe qPCR mix kit Vazyme CatQN213-01 Deposited data Metabolomics data This paper Mendeley data: https://data.mendeley. com/datasets/hy5v62xxpm/2 RNA sequencing This paper SRA: SRP668579 Experimental models: Cell lines HL-60 ATCC Cat#CCL-240 HUVEC ATCC Cat#CRL-1730 Experimental models: Organisms/strains Bama mini pigs Hubei Aofei Biotechnology N/A C57BL/6 mice Hubei Biont Biological Technology N/A Oligonucleotides Primers for qPCR This paper N/A siRNA sequence This paper N/A Recombinant DNA pcDNA3.1-ZNF460 GeneChem N/A pcDNA3.1-GGT1 GeneChem N/A pcDNA3.1-KLF9 GeneChem N/A pcDNA3.1-PGC1α GeneChem N/A Software and algorithms AutoDock Vina Scripps Version 1.1.2 Cutadapt Illumina Version 1.15 FlowJo BD Biosciences Version 10.8.1 GraphPad Prism GraphPad Version 9.5.1 ImageJ NIH Version 1.54f OriginPro 2025 OriginLab Version 10.2.0.188 Pymol Schrödinger Version 2.3.4 Cell Reports Medicine 7, 102714, April 21, 2026 e3 Article ll OPEN ACCESS Medical College, Huazhong University of Science and Technology (Permit number: UHCT-IEC-SOP-016-03-01) and carried out in accordance with the Declaration of Helsinki. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU cell proliferation kit Beyotime Cat#C0078S FerroOrange assay kit Dojindo Cat#F374 Hiscript III reverse transcriptase kit Vazyme Cat#R302-01 Matrigel matrix Corning Cat#356234 Mitochondrial membrane potential assay kit Beyotime Cat#C2006 Mouse TNF-α ELISA kit Boster Cat#EK0527 Mouse IL-4 ELISA kit Boster Cat#EK0405 Mouse IFN-γ ELISA kit Boster Cat#EK0375 Mouse CCL5 ELISA kit NeoBioscience Cat#EMC106 Mouse CCL22 ELISA kit NeoBioscience Cat#EMC105 Mouse bone marrow neutrophil isolation kit Tbdscience Cat#TBD2013NM Nuclear and cytoplasmic protein extraction kit Beyotime Cat#P0027 Reactive oxygen species assay kit Beyotime Cat#S0033S Taurine detection assay kit Cell Biolabs Cat#MET-5071 Taq Pro U+ multiple probe qPCR mix kit Vazyme CatQN213-01 Deposited data Metabolomics data This paper Mendeley data: https://data.mendeley. com/datasets/hy5v62xxpm/2 RNA sequencing This paper SRA: SRP668579 Experimental models: Cell lines HL-60 ATCC Cat#CCL-240 HUVEC ATCC Cat#CRL-1730 Experimental models: Organisms/strains Bama mini pigs Hubei Aofei Biotechnology N/A C57BL/6 mice Hubei Biont Biological Technology N/A Oligonucleotides Primers for qPCR This paper N/A siRNA sequence This paper N/A Recombinant DNA pcDNA3.1-ZNF460 GeneChem N/A pcDNA3.1-GGT1 GeneChem N/A pcDNA3.1-KLF9 GeneChem N/A pcDNA3.1-PGC1α GeneChem N/A Software and algorithms AutoDock Vina Scripps Version 1.1.2 Cutadapt Illumina Version 1.15 FlowJo BD Biosciences Version 10.8.1 GraphPad Prism GraphPad Version 9.5.1 ImageJ NIH Version 1.54f OriginPro 2025 OriginLab Version 10.2.0.188 Pymol Schrödinger Version 2.3.4 Cell Reports Medicine 7, 102714, April 21, 2026 e3 Article ll OPEN ACCESS Medical College, Huazhong University of Science and Technology (Permit number: UHCT-IEC-SOP-016-03-01) and carried out in accordance with the Declaration of Helsinki. ..

    Isolation:

    Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU cell proliferation kit Beyotime Cat#C0078S FerroOrange assay kit Dojindo Cat#F374 Hiscript III reverse transcriptase kit Vazyme Cat#R302-01 Matrigel matrix Corning Cat#356234 Mitochondrial membrane potential assay kit Beyotime Cat#C2006 Mouse TNF-α ELISA kit Boster Cat#EK0527 Mouse IL-4 ELISA kit Boster Cat#EK0405 Mouse IFN-γ ELISA kit Boster Cat#EK0375 Mouse CCL5 ELISA kit NeoBioscience Cat#EMC106 Mouse CCL22 ELISA kit NeoBioscience Cat#EMC105 Mouse bone marrow neutrophil isolation kit Tbdscience Cat#TBD2013NM Nuclear and cytoplasmic protein extraction kit Beyotime Cat#P0027 Reactive oxygen species assay kit Beyotime Cat#S0033S Taurine detection assay kit Cell Biolabs Cat#MET-5071 Taq Pro U+ multiple probe qPCR mix kit Vazyme CatQN213-01 Deposited data Metabolomics data This paper Mendeley data: https://data.mendeley. com/datasets/hy5v62xxpm/2 RNA sequencing This paper SRA: SRP668579 Experimental models: Cell lines HL-60 ATCC Cat#CCL-240 HUVEC ATCC Cat#CRL-1730 Experimental models: Organisms/strains Bama mini pigs Hubei Aofei Biotechnology N/A C57BL/6 mice Hubei Biont Biological Technology N/A Oligonucleotides Primers for qPCR This paper N/A siRNA sequence This paper N/A Recombinant DNA pcDNA3.1-ZNF460 GeneChem N/A pcDNA3.1-GGT1 GeneChem N/A pcDNA3.1-KLF9 GeneChem N/A pcDNA3.1-PGC1α GeneChem N/A Software and algorithms AutoDock Vina Scripps Version 1.1.2 Cutadapt Illumina Version 1.15 FlowJo BD Biosciences Version 10.8.1 GraphPad Prism GraphPad Version 9.5.1 ImageJ NIH Version 1.54f OriginPro 2025 OriginLab Version 10.2.0.188 Pymol Schrödinger Version 2.3.4 Cell Reports Medicine 7, 102714, April 21, 2026 e3 Article ll OPEN ACCESS Medical College, Huazhong University of Science and Technology (Permit number: UHCT-IEC-SOP-016-03-01) and carried out in accordance with the Declaration of Helsinki. ..

    Protein Extraction:

    Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU cell proliferation kit Beyotime Cat#C0078S FerroOrange assay kit Dojindo Cat#F374 Hiscript III reverse transcriptase kit Vazyme Cat#R302-01 Matrigel matrix Corning Cat#356234 Mitochondrial membrane potential assay kit Beyotime Cat#C2006 Mouse TNF-α ELISA kit Boster Cat#EK0527 Mouse IL-4 ELISA kit Boster Cat#EK0405 Mouse IFN-γ ELISA kit Boster Cat#EK0375 Mouse CCL5 ELISA kit NeoBioscience Cat#EMC106 Mouse CCL22 ELISA kit NeoBioscience Cat#EMC105 Mouse bone marrow neutrophil isolation kit Tbdscience Cat#TBD2013NM Nuclear and cytoplasmic protein extraction kit Beyotime Cat#P0027 Reactive oxygen species assay kit Beyotime Cat#S0033S Taurine detection assay kit Cell Biolabs Cat#MET-5071 Taq Pro U+ multiple probe qPCR mix kit Vazyme CatQN213-01 Deposited data Metabolomics data This paper Mendeley data: https://data.mendeley. com/datasets/hy5v62xxpm/2 RNA sequencing This paper SRA: SRP668579 Experimental models: Cell lines HL-60 ATCC Cat#CCL-240 HUVEC ATCC Cat#CRL-1730 Experimental models: Organisms/strains Bama mini pigs Hubei Aofei Biotechnology N/A C57BL/6 mice Hubei Biont Biological Technology N/A Oligonucleotides Primers for qPCR This paper N/A siRNA sequence This paper N/A Recombinant DNA pcDNA3.1-ZNF460 GeneChem N/A pcDNA3.1-GGT1 GeneChem N/A pcDNA3.1-KLF9 GeneChem N/A pcDNA3.1-PGC1α GeneChem N/A Software and algorithms AutoDock Vina Scripps Version 1.1.2 Cutadapt Illumina Version 1.15 FlowJo BD Biosciences Version 10.8.1 GraphPad Prism GraphPad Version 9.5.1 ImageJ NIH Version 1.54f OriginPro 2025 OriginLab Version 10.2.0.188 Pymol Schrödinger Version 2.3.4 Cell Reports Medicine 7, 102714, April 21, 2026 e3 Article ll OPEN ACCESS Medical College, Huazhong University of Science and Technology (Permit number: UHCT-IEC-SOP-016-03-01) and carried out in accordance with the Declaration of Helsinki. ..

    Detection Assay:

    Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU cell proliferation kit Beyotime Cat#C0078S FerroOrange assay kit Dojindo Cat#F374 Hiscript III reverse transcriptase kit Vazyme Cat#R302-01 Matrigel matrix Corning Cat#356234 Mitochondrial membrane potential assay kit Beyotime Cat#C2006 Mouse TNF-α ELISA kit Boster Cat#EK0527 Mouse IL-4 ELISA kit Boster Cat#EK0405 Mouse IFN-γ ELISA kit Boster Cat#EK0375 Mouse CCL5 ELISA kit NeoBioscience Cat#EMC106 Mouse CCL22 ELISA kit NeoBioscience Cat#EMC105 Mouse bone marrow neutrophil isolation kit Tbdscience Cat#TBD2013NM Nuclear and cytoplasmic protein extraction kit Beyotime Cat#P0027 Reactive oxygen species assay kit Beyotime Cat#S0033S Taurine detection assay kit Cell Biolabs Cat#MET-5071 Taq Pro U+ multiple probe qPCR mix kit Vazyme CatQN213-01 Deposited data Metabolomics data This paper Mendeley data: https://data.mendeley. com/datasets/hy5v62xxpm/2 RNA sequencing This paper SRA: SRP668579 Experimental models: Cell lines HL-60 ATCC Cat#CCL-240 HUVEC ATCC Cat#CRL-1730 Experimental models: Organisms/strains Bama mini pigs Hubei Aofei Biotechnology N/A C57BL/6 mice Hubei Biont Biological Technology N/A Oligonucleotides Primers for qPCR This paper N/A siRNA sequence This paper N/A Recombinant DNA pcDNA3.1-ZNF460 GeneChem N/A pcDNA3.1-GGT1 GeneChem N/A pcDNA3.1-KLF9 GeneChem N/A pcDNA3.1-PGC1α GeneChem N/A Software and algorithms AutoDock Vina Scripps Version 1.1.2 Cutadapt Illumina Version 1.15 FlowJo BD Biosciences Version 10.8.1 GraphPad Prism GraphPad Version 9.5.1 ImageJ NIH Version 1.54f OriginPro 2025 OriginLab Version 10.2.0.188 Pymol Schrödinger Version 2.3.4 Cell Reports Medicine 7, 102714, April 21, 2026 e3 Article ll OPEN ACCESS Medical College, Huazhong University of Science and Technology (Permit number: UHCT-IEC-SOP-016-03-01) and carried out in accordance with the Declaration of Helsinki. ..

    RNA Sequencing:

    Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU cell proliferation kit Beyotime Cat#C0078S FerroOrange assay kit Dojindo Cat#F374 Hiscript III reverse transcriptase kit Vazyme Cat#R302-01 Matrigel matrix Corning Cat#356234 Mitochondrial membrane potential assay kit Beyotime Cat#C2006 Mouse TNF-α ELISA kit Boster Cat#EK0527 Mouse IL-4 ELISA kit Boster Cat#EK0405 Mouse IFN-γ ELISA kit Boster Cat#EK0375 Mouse CCL5 ELISA kit NeoBioscience Cat#EMC106 Mouse CCL22 ELISA kit NeoBioscience Cat#EMC105 Mouse bone marrow neutrophil isolation kit Tbdscience Cat#TBD2013NM Nuclear and cytoplasmic protein extraction kit Beyotime Cat#P0027 Reactive oxygen species assay kit Beyotime Cat#S0033S Taurine detection assay kit Cell Biolabs Cat#MET-5071 Taq Pro U+ multiple probe qPCR mix kit Vazyme CatQN213-01 Deposited data Metabolomics data This paper Mendeley data: https://data.mendeley. com/datasets/hy5v62xxpm/2 RNA sequencing This paper SRA: SRP668579 Experimental models: Cell lines HL-60 ATCC Cat#CCL-240 HUVEC ATCC Cat#CRL-1730 Experimental models: Organisms/strains Bama mini pigs Hubei Aofei Biotechnology N/A C57BL/6 mice Hubei Biont Biological Technology N/A Oligonucleotides Primers for qPCR This paper N/A siRNA sequence This paper N/A Recombinant DNA pcDNA3.1-ZNF460 GeneChem N/A pcDNA3.1-GGT1 GeneChem N/A pcDNA3.1-KLF9 GeneChem N/A pcDNA3.1-PGC1α GeneChem N/A Software and algorithms AutoDock Vina Scripps Version 1.1.2 Cutadapt Illumina Version 1.15 FlowJo BD Biosciences Version 10.8.1 GraphPad Prism GraphPad Version 9.5.1 ImageJ NIH Version 1.54f OriginPro 2025 OriginLab Version 10.2.0.188 Pymol Schrödinger Version 2.3.4 Cell Reports Medicine 7, 102714, April 21, 2026 e3 Article ll OPEN ACCESS Medical College, Huazhong University of Science and Technology (Permit number: UHCT-IEC-SOP-016-03-01) and carried out in accordance with the Declaration of Helsinki. ..

    Sequencing:

    Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU cell proliferation kit Beyotime Cat#C0078S FerroOrange assay kit Dojindo Cat#F374 Hiscript III reverse transcriptase kit Vazyme Cat#R302-01 Matrigel matrix Corning Cat#356234 Mitochondrial membrane potential assay kit Beyotime Cat#C2006 Mouse TNF-α ELISA kit Boster Cat#EK0527 Mouse IL-4 ELISA kit Boster Cat#EK0405 Mouse IFN-γ ELISA kit Boster Cat#EK0375 Mouse CCL5 ELISA kit NeoBioscience Cat#EMC106 Mouse CCL22 ELISA kit NeoBioscience Cat#EMC105 Mouse bone marrow neutrophil isolation kit Tbdscience Cat#TBD2013NM Nuclear and cytoplasmic protein extraction kit Beyotime Cat#P0027 Reactive oxygen species assay kit Beyotime Cat#S0033S Taurine detection assay kit Cell Biolabs Cat#MET-5071 Taq Pro U+ multiple probe qPCR mix kit Vazyme CatQN213-01 Deposited data Metabolomics data This paper Mendeley data: https://data.mendeley. com/datasets/hy5v62xxpm/2 RNA sequencing This paper SRA: SRP668579 Experimental models: Cell lines HL-60 ATCC Cat#CCL-240 HUVEC ATCC Cat#CRL-1730 Experimental models: Organisms/strains Bama mini pigs Hubei Aofei Biotechnology N/A C57BL/6 mice Hubei Biont Biological Technology N/A Oligonucleotides Primers for qPCR This paper N/A siRNA sequence This paper N/A Recombinant DNA pcDNA3.1-ZNF460 GeneChem N/A pcDNA3.1-GGT1 GeneChem N/A pcDNA3.1-KLF9 GeneChem N/A pcDNA3.1-PGC1α GeneChem N/A Software and algorithms AutoDock Vina Scripps Version 1.1.2 Cutadapt Illumina Version 1.15 FlowJo BD Biosciences Version 10.8.1 GraphPad Prism GraphPad Version 9.5.1 ImageJ NIH Version 1.54f OriginPro 2025 OriginLab Version 10.2.0.188 Pymol Schrödinger Version 2.3.4 Cell Reports Medicine 7, 102714, April 21, 2026 e3 Article ll OPEN ACCESS Medical College, Huazhong University of Science and Technology (Permit number: UHCT-IEC-SOP-016-03-01) and carried out in accordance with the Declaration of Helsinki. ..

    Recombinant:

    Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU cell proliferation kit Beyotime Cat#C0078S FerroOrange assay kit Dojindo Cat#F374 Hiscript III reverse transcriptase kit Vazyme Cat#R302-01 Matrigel matrix Corning Cat#356234 Mitochondrial membrane potential assay kit Beyotime Cat#C2006 Mouse TNF-α ELISA kit Boster Cat#EK0527 Mouse IL-4 ELISA kit Boster Cat#EK0405 Mouse IFN-γ ELISA kit Boster Cat#EK0375 Mouse CCL5 ELISA kit NeoBioscience Cat#EMC106 Mouse CCL22 ELISA kit NeoBioscience Cat#EMC105 Mouse bone marrow neutrophil isolation kit Tbdscience Cat#TBD2013NM Nuclear and cytoplasmic protein extraction kit Beyotime Cat#P0027 Reactive oxygen species assay kit Beyotime Cat#S0033S Taurine detection assay kit Cell Biolabs Cat#MET-5071 Taq Pro U+ multiple probe qPCR mix kit Vazyme CatQN213-01 Deposited data Metabolomics data This paper Mendeley data: https://data.mendeley. com/datasets/hy5v62xxpm/2 RNA sequencing This paper SRA: SRP668579 Experimental models: Cell lines HL-60 ATCC Cat#CCL-240 HUVEC ATCC Cat#CRL-1730 Experimental models: Organisms/strains Bama mini pigs Hubei Aofei Biotechnology N/A C57BL/6 mice Hubei Biont Biological Technology N/A Oligonucleotides Primers for qPCR This paper N/A siRNA sequence This paper N/A Recombinant DNA pcDNA3.1-ZNF460 GeneChem N/A pcDNA3.1-GGT1 GeneChem N/A pcDNA3.1-KLF9 GeneChem N/A pcDNA3.1-PGC1α GeneChem N/A Software and algorithms AutoDock Vina Scripps Version 1.1.2 Cutadapt Illumina Version 1.15 FlowJo BD Biosciences Version 10.8.1 GraphPad Prism GraphPad Version 9.5.1 ImageJ NIH Version 1.54f OriginPro 2025 OriginLab Version 10.2.0.188 Pymol Schrödinger Version 2.3.4 Cell Reports Medicine 7, 102714, April 21, 2026 e3 Article ll OPEN ACCESS Medical College, Huazhong University of Science and Technology (Permit number: UHCT-IEC-SOP-016-03-01) and carried out in accordance with the Declaration of Helsinki. ..

    Software:

    Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER EdU cell proliferation kit Beyotime Cat#C0078S FerroOrange assay kit Dojindo Cat#F374 Hiscript III reverse transcriptase kit Vazyme Cat#R302-01 Matrigel matrix Corning Cat#356234 Mitochondrial membrane potential assay kit Beyotime Cat#C2006 Mouse TNF-α ELISA kit Boster Cat#EK0527 Mouse IL-4 ELISA kit Boster Cat#EK0405 Mouse IFN-γ ELISA kit Boster Cat#EK0375 Mouse CCL5 ELISA kit NeoBioscience Cat#EMC106 Mouse CCL22 ELISA kit NeoBioscience Cat#EMC105 Mouse bone marrow neutrophil isolation kit Tbdscience Cat#TBD2013NM Nuclear and cytoplasmic protein extraction kit Beyotime Cat#P0027 Reactive oxygen species assay kit Beyotime Cat#S0033S Taurine detection assay kit Cell Biolabs Cat#MET-5071 Taq Pro U+ multiple probe qPCR mix kit Vazyme CatQN213-01 Deposited data Metabolomics data This paper Mendeley data: https://data.mendeley. com/datasets/hy5v62xxpm/2 RNA sequencing This paper SRA: SRP668579 Experimental models: Cell lines HL-60 ATCC Cat#CCL-240 HUVEC ATCC Cat#CRL-1730 Experimental models: Organisms/strains Bama mini pigs Hubei Aofei Biotechnology N/A C57BL/6 mice Hubei Biont Biological Technology N/A Oligonucleotides Primers for qPCR This paper N/A siRNA sequence This paper N/A Recombinant DNA pcDNA3.1-ZNF460 GeneChem N/A pcDNA3.1-GGT1 GeneChem N/A pcDNA3.1-KLF9 GeneChem N/A pcDNA3.1-PGC1α GeneChem N/A Software and algorithms AutoDock Vina Scripps Version 1.1.2 Cutadapt Illumina Version 1.15 FlowJo BD Biosciences Version 10.8.1 GraphPad Prism GraphPad Version 9.5.1 ImageJ NIH Version 1.54f OriginPro 2025 OriginLab Version 10.2.0.188 Pymol Schrödinger Version 2.3.4 Cell Reports Medicine 7, 102714, April 21, 2026 e3 Article ll OPEN ACCESS Medical College, Huazhong University of Science and Technology (Permit number: UHCT-IEC-SOP-016-03-01) and carried out in accordance with the Declaration of Helsinki. ..



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